LSM









General

Zeiss LSM 710 meta | acquired 2011

Resources

Introduction booklet: download

Laserlines

| 458 | 488 | 514 |  | 633

Objectives

  • Plan-Apochromat 10x/0.3 M27
  • Plan-Apochromat 20x/0.8 M27
  • LCI Plan-Neofluar 25x/0.8 Imm Korr DIC M27
  • EC Plan-Neofluar 40x/1.30 Oil DIC M27
  • Plan-Apochromat 63x/1.4 Oil M27

Meta filter

Customizable spectrometer between 400 nm and 700 nm

Environmental

Incubator Peacon XLmulti S1 | Heating unit XL S1 | CO2 unit S1

Training

Note that room C0141 is scheduled for cleaning every Friday from 18:00 to 20:00. Bookings during this time slot should be avoided.

  1. A Request for introduction by the responsible admin is mandatory.
  2. Only persons who received the introduction are eligible to use the instrument.  The introduction is  personal and non-transferable: it does not legitimate other members of his/her group and/or colleagues to use the instrument.
  3. The Instrument is available on weekdays from 9:00 am to 5:00 pm. For other access
  4. Reservation via the booking platform is mandatory.
  5. Reservations cannot be placed more than 2 weeks (14 days) in advance.
  6. Cancellations must be announced at least 24 hours in advance, in order to give other users a chance to use the opened time slot.
  7. Early finished: inform the user that is coming after you (email and phone in the booking)
  8. Before starting the session, the user must check the instrument and report any damage or incorrect state immediately to the Responsible.

Troubleshooting

Immersion oil

Which immersion oil to use?

Oil / Application Brightfield Fluorescence Live cell imaging Note
Type B yes no no
Type 37 yes no yes
Type HF yes yes yes Halogen-free
Type LDF yes yes yes Contains halogens
Type FF yes yes no

Contact

Dimitri Vanhecke | tel:  9509 | @ dimitri.vanhecke@unifr.ch
Mauro Almeida | tel:  9592 | @ mauro.sousadealmeida@unifr.ch